LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2552..2648) /label=attL2 /note="attL2 (c)" misc_signal complement(2663..2682) /note="T7 Promoter/priming site (c)" primer_bind 2690..2706 /note="M13 Reverse priming site" gene 2819..3628 /note="Kanamycin resistance gene" rep_origin 3749..4422 /note="pUC origin" vector join(2560..4425,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2545 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:57606 IMAGE:4442080" /tissue_type="Liver, adenocarcinoma" /clone_lib="NIH_MGC_90" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 692..2545 /gene="GALNT7" /gene_synonym=GALNAC-T7 /gene_synonym=GalNAcT7 /db_xref="GeneID:51809" /db_xref="HGNC:4129" /db_xref="MIM:605005" CDS 692..2545 /dnas_title="UDP-N-acetyl-alpha-D-galactosamine:polypeptid e N-acetylgalactosaminyltransferase 7 (GalNAc-T7)" /gene="GALNT7" /gene_synonym=GALNAC-T7 /gene_synonym=GalNAcT7 /codon_start=1 /product="UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7 (GalNAc-T7)" /protein_id="AAH46129.1" /db_xref="GI:28279289" /db_xref="GeneID:51809" /db_xref="HGNC:4129" /db_xref="MIM:605005" /translation="MRLKIGFILRSLLVVGSFLGLVVLWSSLTPRPDDPSPLSRMRED RDVNDPMPNRGGNGLAPGEDRFKPVVPWPHVEGVEVDLESIRRINKAKNEQEHHAGGD SQKDIMQRQYLTFKPQTFTYHDPVLRPGILGNFEPKEPEPPGVVGGPGEKAKPLVLGP EFKQAIQASIKEFGFNMVASDMISLDRSVNDLRQEECKYWHYDENLLTSSVVIVFHNE GWSTLMRTVHSVIKRTPRKYLAEIVLIDDFSNKEHLKEKLDEYIKLWNGLVKVFRNER REGLIQARSIGAQKAKLGQVLIYLDAHCEVAVNWYAPLVAPISKDRTICTVPLIDVIN GNTYEIIPQGGGDEDGYARGAWDWSMLWKRVPLTPQEKRLRKTKTEPYRSPAMAGGLF AIEREFFFELGLYDPGLQIWGGENFEISYKIWQCGGKLLFVPCSRVGHIYRLEGWQGN PPPIYVGSSPTLKNYVRVVEVWWDEYKDYFYASRPESQALPYGDISELKKFREDHNCK SFKWFMEEIAYDITSHYPLPPKNVDWGEIRGFETAYCIDSMGKTNGGFVELGPCHRMG GNQLFRINEANQLMQYDQCLTKGADGSKVMITHCNLNEFKEWQYFKNLHRFTHIPSGK CLDRSEVLHQVFISNCDSSKTTQKWEMNNIHSV" misc_feature 692..2545 /note="GALNT7 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg caggatgagg gaagacagag atgtcaatga 721 ccccatgccc aaccgaggcg gcaatggact agctcctggg gaggacagat tcaaacctgt 781 ggtaccatgg cctcatgttg aaggagtaga agtggactta gagtctatta gaagaataaa 841 caaggccaaa aatgaacaag agcaccatgc tggaggagat tcccagaaag atatcatgca 901 gaggcagtat ctcacattta agcctcagac attcacctac catgatcctg tgcttcgccc 961 agggatcctc ggtaactttg aacccaaaga acctgagcct cctggagtgg ttggtggccc 1021 tggagagaaa gccaagccat tggttttggg accagaattc aaacaagcaa ttcaagccag 1081 cattaaagag tttggattta acatggtggc aagtgacatg atctcactgg accgcagcgt 1141 caatgactta cgccaagaag aatgcaagta ttggcattat gatgaaaact tgctcacttc 1201 gagcgttgtc attgtcttcc ataatgaagg atggtcaacc ctcatgagaa cagtccacag 1261 tgtaattaaa aggactccaa ggaaatattt agcagaaatt gtgttaattg acgatttcag 1321 taataaagaa cacttaaaag aaaaactgga tgaatatatt aagctgtgga atggcctagt 1381 gaaggtattt cgaaatgaaa gaagggaagg tttaattcaa gcacgaagta ttggtgctca 1441 gaaggctaaa cttggacagg ttttgatata ccttgatgcc cactgtgagg tggcagttaa 1501 ctggtatgca ccacttgtag ctcccatatc taaggacaga accatttgca ctgtgccgct 1561 tatagatgtc ataaatggca acacatatga aattataccc caagggggtg gtgatgaaga 1621 tgggtatgcc cgaggagcat gggattggag tatgctctgg aaacgggtgc ctctgacccc 1681 tcaagagaag agactgagaa agacaaaaac tgaaccgtat cggtccccag ccatggctgg 1741 gggattattt gccattgaac gagagttctt ctttgaattg ggtctctatg atccaggtct 1801 ccagatttgg ggtggtgaaa actttgagat ctcatacaag atatggcagt gtggtggcaa 1861 attattattt gttccttgtt ctcgtgttgg acatatctac cgtcttgagg gctggcaagg 1921 aaatcctccg cccatttatg ttgggtcttc tccaactctg aagaattatg ttagagttgt 1981 ggaggtttgg tgggatgaat ataaagacta cttctatgct agtcgtcctg aatcgcaggc 2041 attaccatat ggggatatat cggagctgaa aaaatttcga gaagatcaca actgcaaaag 2101 ttttaagtgg ttcatggaag aaatagctta tgatatcacc tcacactacc ctttgccacc 2161 caaaaatgtt gactggggag aaatcagagg cttcgaaact gcttactgca ttgatagcat 2221 gggaaaaaca aatggaggct ttgttgaact aggaccctgc cacaggatgg gagggaatca 2281 gcttttcaga atcaatgaag caaatcaact catgcagtat gaccagtgtt tgacaaaggg 2341 agctgatgga tcaaaagtta tgattacaca ctgtaatcta aatgaattta aggaatggca 2401 gtacttcaag aacctgcaca gatttactca tattccttca ggaaagtgtt tagatcgctc 2461 agaggtcctg catcaagtat tcatctccaa ttgtgactcc agtaaaacga ctcaaaaatg 2521 ggaaatgaat aacatccata gtgttTAGGA CCCAGCTTTC TTGTACAAAG TTGGCATTAT 2581 AAGAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC ACTATCAGTC AAAATAAAAT 2641 CATTATTTGC CATCCAGCTG ATATCCCCTA TAGTGAGTCG TATTACATGG TCATAGCTGT 2701 TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA TCTCTGATGT TACATTGCAC AAGATAAAAT 2761 AATATCATCA TGAACAATAA AACTGTCTGC TTACATAAAC AGTAATACAA GGGGTGTTAT 2821 GAGCCATATT CAACGGGAAA CGTCGAGGCC GCGATTAAAT TCCAACATGG ATGCTGATTT 2881 ATATGGGTAT AAATGGGCTC GCGATAATGT CGGGCAATCA GGTGCGACAA TCTATCGCTT 2941 GTATGGGAAG CCCGATGCGC CAGAGTTGTT TCTGAAACAT GGCAAAGGTA GCGTTGCCAA 3001 TGATGTTACA GATGAGATGG TCAGACTAAA CTGGCTGACG GAATTTATGC CTCTTCCGAC 3061 CATCAAGCAT TTTATCCGTA CTCCTGATGA TGCATGGTTA CTCACCACTG CGATCCCCGG 3121 AAAAACAGCA TTCCAGGTAT TAGAAGAATA TCCTGATTCA GGTGAAAATA TTGTTGATGC 3181 GCTGGCAGTG TTCCTGCGCC GGTTGCATTC GATTCCTGTT TGTAATTGTC CTTTTAACAG 3241 CGATCGCGTA TTTCGTCTCG CTCAGGCGCA ATCACGAATG AATAACGGTT TGGTTGATGC 3301 GAGTGATTTT GATGACGAGC GTAATGGCTG GCCTGTTGAA CAAGTCTGGA AAGAAATGCA 3361 TAAACTTTTG CCATTCTCAC CGGATTCAGT CGTCACTCAT GGTGATTTCT CACTTGATAA 3421 CCTTATTTTT GACGAGGGGA AATTAATAGG TTGTATTGAT GTTGGACGAG TCGGAATCGC 3481 AGACCGATAC CAGGATCTTG CCATCCTATG GAACTGCCTC GGTGAGTTTT CTCCTTCATT 3541 ACAGAAACGG CTTTTTCAAA AATATGGTAT TGATAATCCT GATATGAATA AATTGCAGTT 3601 TCATTTGATG CTCGATGAGT TTTTCTAATC AGAATTGGTT AATTGGTTGT AACACTGGCA 3661 GAGCATTACG CTGACTTGAC GGGACGGCGC AAGCTCATGA CCAAAATCCC TTAACGTGAG 3721 TTACGCGTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA 3781 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT 3841 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG 3901 AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA 3961 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG 4021 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA 4081 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC 4141 CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA 4201 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC 4261 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG 4321 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC 4381 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTT //